Methods: This research enrolls 20 healthy pregnant white women, 18-35 years of age, with normal body mass index (BMI), and who present for prenatal care at a birth center in the Southwest United States. Women enrolled in this prospective study have cervical specimens collected at 2 points: at 35-37 weeks and at the onset of labor. Where the participant has a Cesarean birth, a myometrial specimen is obtained to provide preliminary data regarding microbiota community taxonomic composition and diversity and how that compares with that found in the cervix. A standardized collection and processing model is being used to swab the cervix with preservation of samples by immediate freezing. Demographics, baseline clinical characteristics, and select perinatal outcomes are examined in relation to the community of microbes noted in the cervix. Dietary intake (24 hour recall) - a select host factor is also examined for consideration of potential impact on noted microbiota and associated perinatal outcomes.
Results: Data will be analyzed to characterize the cervical and uterine microbial communities and determination of correlations between differences in the microbiome and perinatal outcomes with microbiome compositions being measured by 16S rRNA gene sequencing.
Conclusion: The goal of this research is to promote health in pregnancy and improve perinatal outcomes. Findings have the potential for further refinement of personalized medicine for pregnant women, including development of biomarkers which could be key in the development of targeted interventions capable of promoting or blocking parturition at less desirable endpoints.