Methods: This is a prospective randomized controlled trial. Level III neonatal intensive care unit and a neonatal unit at a medical center in Taipei. Preterm infants (N=70, gestational age 28–37 weeks, stable disease condition) needing procedural venous punctures were recruited by convenience sampling and randomly assigned to two treatment conditions: routine care, breast milk odor (BMO) + oral expressed breast milk (OEBM). Pain was measured by watching video recordings of infants undergoing venipuncture procedures and scoring pain at 1-minute intervals with the Premature Infant Pain Profile-Revised; physiological parameters (heart rate [HR], oxygen saturation [SpO2]) by electrocardiogram monitors, and was be digitally sampled at 10-s intervals by computer. Data were collected over nine phases: baseline (phase 0, 5 min without stimuli before venipuncture), disinfecting (phase 1), during venipuncture (phase 2), and a 10-minute recovery (phases 3-8).
Results: For the group of the infants receiving BMO+ OEBM, pain score changes from baseline were 1.90, 2.15, and 2.00 units significantly lower than corresponding pain-score changes in infants receiving routine care across phases 2, 5, 6 ( p-values < 0.05, and p-values=0.05 ). However, there were no significant differences in pain score changes between the two groups at phase 3, 4, 7, and 8. For the group of the infants receiving BMO+ OEBM, HR changes from baseline across phase 2,5, 8 were 8.00, 8.88, 5.25 units significantly lower than corresponding HR changes of infants receiving routine care ( all p-values < 0.05). However, there were no significant differences in HR changes between the two groups at phase 1, 3, 4, 6, and 7. For the group of the infants receiving BMO+ OEBM, the SpO2 changes from baseline across phases 4 were 2.49 units higher than corresponding SpO2 changes of infants receiving routine care ( p-values < 0.05). However, there were no significant differences in SpO2 changes between the two groups at phase 1, 2, 3, 5, 6, 7 and 8.
Conclusion: The study suggests that preterm infants receiving BMO+ OEBM could significantly lower the infant’s pain-score, and lower the changes of the HR during the venipuncture phase. The results can guide caregivers to alleviate preterm infants’ pain during painful procedure by using breast milk odor/taste integration. By using the infant’s sensory competences; clinicians could calm their pain, and HR across the venipuncture phase.