Sandra K. Hanneman, RN, PhD, FAAN1, Kortney E. Green, BA1, and Nikhil S. Padhye, PhD2. (1) Center for Nursing Research, University of Texas School of Nursing at Houston, Houston, Texas, USA, (2) Center for Nursing Research, University of Texas Health Science Center at Houston, Houston, Texas, USA
Many ethnic/racial
health disparities raise questions about differences in stress hormone
secretion. Ethnicity and educational level have been found to affect salivary
awakening cortisol levels. Salivary cortisol reflects the biologically active unbound (free)
fraction of the circulating hormone, and correlates strongly with plasma free cortisol. Salivary cortisol was
collected from 23 doctoral nursing students and instructors and analyzed by
enzyme immunoassay (Salimetrics LLC, State College, PA).
Subjects were instructed to withhold food, drink, smoking and oral hygiene for
1 hr and alcohol consumption for 12 hr before sampling. Cotton balls were held
in the mouth until saturated; saliva was expressed into collection tubes and
immediately assayed in duplicate. Optical density was measured with a microtitration plate reader (VICTOR3 Multilabel Plate Reader, PerkinElmer, Shelton, NY)
with a 450 nm filter. Unknown cortisol concentrations
were computed from optical absorption values using linear calibration on cortisol standards in a log-logit
transformed domain. Subjects were predominantly female (21/23) and students
(19/23); 14 (64%) were Caucasian, 6 (27%) Asian, 1 (5%) Hispanic and 1 (5%) East Indian. Cortisol
concentration varied from .113 – 1.243 μg/dl (M
= 0.47 ± 0.34). Duplicate measures were highly correlated (r = 0.87, P <
0.001); intra-assay coefficients of variation were 9% for controls and 37% for
the sample. Mean (± SD) cortisol was .30 ± .22 for
Caucasian and .86 ± .25 for Asian subjects, with significantly higher values in
Asian subjects (F = 9.6; df =
3, 18; P = 0.001). Asian doctoral nursing students appear to have higher
salivary free cortisol concentrations than Caucasian students. Whether these
preliminary findings reflect genetic factors, the stress of living in a foreign
country or a combination thereof remains to be determined. Further study is
needed given the numbers of international students pursuing higher education in
a foreign country.