Paper
Saturday, 22 July 2006
This presentation is part of : Initiatives for Cancer Patients
Pathophysiological Changes Associated with the Exposure of Sustained Delivery of Glucocorticoids on Pituitary Adenoma Cell Line
Lisa A. Haynie, PhD, RN, CFNP, School of Nursing, University of Mississippi Medical Center, Jackson, MS, USA, Hamed A. Benghuzzi, PhD, School of Health-Related Professions, University of Mississippi Medical Center, Jackson, MS, USA, and Michelle A. Tucci, PhD, Orthopedics, University of Mississippi Medical Center, Jackson, MS, USA.
Learning Objective #1: The learner will be able to explain the mechanism of action of glucocorticoids in relation to the hypothalamic-piuitary-adrenal axis (HPA)
Learning Objective #2: The learner will be able to describe how sustained delivery of glucocorticoids will effect RC/4B pituitary adenoma cells.

Glucocorticoid receptor rich tissues such as pituitary cells express tissue specific 11 β hydroxysteroid type 1 enzyme, which causes cortisol to act as an autocrine, anti-proliferative, pro-differentiation stimulus in normal tissue. In pituitary tumor cells there is a strong correlation indicating a shift from 11 β hydroxysteroid type 1 enzyme activity to 11 β hydroxysteroid type 2 enzyme activity. Shifting causes an anti-proliferative effect, which may be effective in suppressing tumor activity. Overall, the results indicated a decreased in cell number for both acute and chronic administration over a 96 hour period without inducing cellular damage (MDA), reactive nitric intermediates (nitric oxide) or reducing cellular antioxidant status (glutathione). Cortisol administration caused an increase in the number of hyperchromic nuclei suggesting cell cycle regulation. These findings conclude that cortisol administration to pituitary tumor cells may offer a future means of treatment for patients post hypophysectomy.

See more of Initiatives for Cancer Patients
See more of The 17th International Nursing Research Congress Focusing on Evidence-Based Practice (19-22 July 2006)