Poster Presentation

Monday, November 5, 2007
10:30 AM - 11:45 AM

Monday, November 5, 2007
1:30 PM - 2:45 PM
This presentation is part of : Scientific Posters
Omega-3 Fatty Acids Effect on Wound Healing
Jodi McDaniel, MS, RN, Karen Ahijevych, PhD, RN, and Wendy Blakely, PhD, RN. College of Nursing, The Ohio State University, Columbus, OH, USA
Learning Objective #1: understand how increasing consumption of omega-3 fatty acids in fish oil supplements may delay wound healing by decreasing pro-inflammatory cytokines.
Learning Objective #2: appreciate the monumental problem of nonhealing wounds in the United States and identify psychoneuroimmunological variables that affect healing.

Purpose:  To examine effects of ω-3 fatty acids eicosapentaenoic (EPA) and docosahexaenoic (DHA), obtained from fish oil, on wound healing. The specific aims were: 1) to compare pro-inflammatory levels at blister sites between healthy individuals receiving ω-3 supplements and a control group; and 2) to compare wound healing between the two groups.  
 It was hypothesized that reducing pro-inflammatory cytokine production through consumption of ω-3 fatty acids could delay the necessary inflammatory stage of healing.
Design and Method:  A prospective, randomized, double-blind, experimental design included Group 1 (n=16), receiving ω-3 fatty supplements for four weeks prior to the blistering procedure and Group 2 (n=14), receiving a placebo for 4 weeks. The independent variable was ω-3 fatty acid supplements, which were biochemically confirmed by plasma per gas chromatography. Dependent variables were the inflammatory stage of healing measured by proinflammatory cytokines via enzyme-linked immunosorbent assay (ELISA) and wound healing determined by single digital camera photogrammetry. 
Pro-inflammatory cytokines were measured in blister serum at 5 and 24 hours post formation of 8 small blisters on nondominant forearm. Salivary cortisol and a Perceived Stress Scale evaluated stress levels, which can affect healing. Wound areas were measured daily until 100% healed.  Subjects completed 3-day food diaries at baseline and four weeks with micronutrients quantified by Nutrition Data System for Research (NDSR).
Preliminary Findings:  Subjects = 30 healthy individuals, 57% women and 43% male, (mean ± SD age, 25.4 ± 6.4 years). Ethnicity = 77% White, 13% Asian and 10% African American. Mean days to healing = 10.6 ± 3.6 days (active subgroup = 11.1 ± 4.4; placebo = 9.8 ± 2.1). Fatty acid, pro-inflammatory cytokine and salivary cortisol assays are in process, as are PSS scores and nutritional analyses.
Conclusions:  Pending the outcome, evidence may encourage discontinuation of supplemental ω-3 fatty acids prior to elective surgery.